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Anti-Mouse IgG F(ab′)2-Peroxidase antibody produced in goat

产品编号:4080664
规格:affinity isolated antibody, lyophilized powder
包装规格:2 MG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40806642 MG3280
产品别名

Anti-Mouse IgG F(ab′)2-Peroxidase antibody produced in goat

HRP

基本信息
NACRES
NA.46
General description【一般描述】
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. Pepsin cleaves the carboxy-terminal side of the disulfide bonds in the general region of the antibody and this gives rise to the F(ab′)2 fragment. The two antigen-binding arms of the antibody are linked in this fragment. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants.
Specificity【特异性】
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Goat Serum, Mouse IgG, Mouse IgG F(ab′)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Immunogen【免疫原】
Mouse IgG F(ab′)2 fragment
Application【应用】
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Physical properties【物理属性】
Antibody format: IgG
Physical form【外形】
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Reconstitution【重悬】
Reconstitute with 1.0 mL deionized water (or equivalent).
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
goat
conjugate【偶联物】
peroxidase conjugate
antibody form【抗体形式】
affinity isolated antibody
antibody product type
secondary antibodies
clone【克隆】
polyclonal
form【形式】
lyophilized powder
species reactivity
mouse
technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable
shipped in【运输】
wet ice
storage temp.【储存温度】
2-8℃
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H317
Precautionary Statements
P280
Hazard Classifications【危险分类】
Skin Sens. 1
Storage Class Code【储存分类代码】
13 - Non Combustible Solids
WGK
WGK 3
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