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氯化四唑氮蓝

Nitrotetrazolium Blue chloride
产品编号:4081394
规格:90.0-110.0% (calc. on dry substance, T)
CAS NO:298-83-9
包装规格:100 MG,500 MG,2.5 G
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品编号包装单位单价(元)国内现货国外库存询价单
4081394100 MG883
4081394500 MG2950
40813942.5 G10680
产品别名

298-83-9

氯化四唑氮蓝

Nitrotetrazolium Blue chloride

3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride

p-Nitrotetrazolium blue

Nitro BT

NBT

p-Nitro-Blue tetrazolium chloride

硝基蓝四氮唑

化硝基四氮唑蓝

硝基蓝四氮唑

四唑蓝

结构式
基本信息
Linear Formula【线性分子式】
C40H30N10O6 · 2Cl
Molecular weight
817.64
Beilstein
4115923
MDL number
MFCD00012159
PubChem Substance ID【PubChem化学物质编号】
329770281
NACRES
NA.54
Biochem/physiol Actions【生化/生理作用】
Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained. It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases. NBT is also used for diagnosing microbial infections and phagocytic defects. In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate for alkaline phosphatase enzyme reaction, in which NBT is reduced to insoluble purple diformazan.
Analysis Note【分析说明】
solubility:
250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow
产品性质
Quality Level【质量水平】
100
Assay【测定】
90.0-110.0% (calc. on dry substance, T)
impurities【杂质】
≤10% solvent content
≤10% water
λ
in methanol
UV absorption【紫外吸收】
λ: 252-262 nm Amax
storage temp.【储存温度】
2-8℃
SMILES string
[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI
1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key
FSVCQIDHPKZJSO-UHFFFAOYSA-L
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H302 - H315 - H319 - H335
Precautionary Statements
P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs【靶器官】
Respiratory system
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 3
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