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Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate
| 产品编号： | 4081786 |
| 规格： | clone TRA-1-81, from mouse, CY3 conjugate |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate
Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | TRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin. |
| Specificity【特异性】 | This antibody recognizes human TRA-1-81. |
| Immunogen【免疫原】 | Human embryonal carcinoma cell line 2102Ep |
| Application【应用】 | Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012). This Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81. Research Sub Category Pluripotent & Early Differentiation Research Category Stem Cell Research |
| Quality【质量】 | Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells. |
| Physical form【外形】 | Ammonium sulfate precipitation followed by size exclusion. Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA. |
| Analysis Note【分析说明】 | Control H9 human embryonic stem cells |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | CY3 conjugate |
| antibody form【抗体形式】 | saturated ammonium sulfate (SAS) precipitated |
| antibody product type | primary antibodies |
| clone【克隆】 | TRA-1-81, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| isotype【同位素/亚型】 | IgM |
| NCBI accession no.【NCBI登记号】 | NP_001018121.1 |
| UniProt accession no.【UniProt登记号】 | O00592 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... PODXL(5420) |
产品说明
| Target description【目标描述】 | 420/250 kDa calculated |
| Storage and Stability【储存及稳定性】 | Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
