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Rosetta-gami 2 Competent Cell Set-Novagen

产品编号:4082043
包装规格:1 SET
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
40820431 SET4500
产品性质
manufacturer/tradename
Novagen®
Quality Level【质量水平】
100
storage condition【储存条件】
OK to freeze
cell transformation【细胞转化】
transformation efficiency: 2×106 cfu/μg
storage temp.【储存温度】
−70℃
基本信息
General description【一般描述】
Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotypes:

Rosetta-gami 2

Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)



Rosetta-gami 2(DE3)

Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR)



Rosetta-gami 2(DE3)pLysS

Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac+ lacIq pro] gor522::Tn10 trxB pLysSRARE2 (CamR, StrR, TetR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Components【组分】
2 × 0.2 mlRosetta-gami 2 Competent Cells

2 × 0.2 mlRosetta-gami 2(DE3) Competent Cells

2 × 0.2 mlRosetta-gami 2(DE3)pLysS Competent Cells

4 × 2 mlSOC Medium

10 µlTest Plasmid

Warning【警告】
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information【法律信息】
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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