您的位置：首页 > 产品中心 > BugBuster® GST-Bind Purification Kit
BugBuster® GST-Bind Purification Kit
| 产品编号： | 4082053 |
| 规格： | Affinity purification of GST fusion proteins |
| 包装规格： | 1 KIT |
| 产品类别： | 进口试剂 |
| 品牌： | Millipore |
| 优惠价： | 立即咨询 |
产品性质
| manufacturer/tradename | Novagen® |
| storage condition【储存条件】 | OK to freeze |
| packaging【包装】 | 1 kit in Glass bottle |
基本信息
| General description【一般描述】 | Affinity purification of GST fusion proteins The BugBuster® GST•Bind<TMSYMBOL></TMSYMBOL> Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag<TMSYMBOL></TMSYMBOL> fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.  | |||||||||||||||||||||
| Components【组分】 | | • | 2 x 100 ml | BugBuster Protein Extraction Reagent | • | 10,000 U | Benzonase Nuclease, purity >90% | • | 10 ml | GST•Bind Resin | • | pkg/4 | Chromatography Columns | • | 2 x 100 ml | 10X GST Bind/Wash Buffer | • | 40 ml | 10X Glutathione Reconstitution Buffer | • | 1 g | Glutathione, Reduced | |
| Warning【警告】 | Toxicity: Multiple Toxicity Values, refer to MSDS (O) | |||||||||||||||||||||
| Legal Information【法律信息】 | BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 3 - Flammable liquids |
