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Rosetta-gami 2 Competent Cells-Novagen
| 产品编号： | 4082105 |
| 规格： | Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. |
| 包装规格： | 1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| Quality Level【质量水平】 | 100 |
| manufacturer/tradename | Novagen® |
| storage condition【储存条件】 | OK to freeze |
| cell transformation【细胞转化】 | transformation efficiency: 2×106 cfu/μg |
| storage temp.【储存温度】 | −70℃ |
基本信息
| General description【一般描述】 | Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs. Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli. | ||||||||||||||||
| Components【组分】 | | 0.4 ml | 1 ml | Component | • | 2 × 0.2 ml | 5 × 0.2 ml | Rosetta-gami 2 Competent Cells | • | 2 × 2 ml | 4 × 2 ml | SOC Medium | • | 10 µl | 10 µl | Test Plasmid | | |
| Warning【警告】 | Toxicity: Multiple Toxicity Values, refer to MSDS (O) | ||||||||||||||||
| Legal Information【法律信息】 | NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany |
