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YeastBuster Protein Extraction Reagent
| 产品编号： | 4082226 |
| 规格： | Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis |
| 包装规格： | 100 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Millipore |
| 优惠价： | 立即咨询 |
产品性质
| manufacturer/tradename | Novagen® |
| storage condition【储存条件】 | OK to freeze |
| shipped in【运输】 | wet ice |
| packaging【包装】 | 100, 500 ml in Plastic ampoule |
基本信息
| General description【一般描述】 |    Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis YeastBuster<TMSYMBOL></TMSYMBOL> Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells. In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes. | ||||||
| Features and Benefits【特点和优势】 |
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| Components【组分】 | | • | 100 ml or 500 ml | YeastBuster | • | 1 ml or 5 ml | 100X THP Solution | |
| Warning【警告】 | Toxicity: Multiple Toxicity Values, refer to MSDS (O) | ||||||
| Other Notes【其他说明】 | Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. | ||||||
| Legal Information【法律信息】 | HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Warning |
| Hazard Statements | H373 |
| Precautionary Statements | P260 - P314 - P501 |
| Hazard Classifications【危险分类】 | STOT RE 2 Oral |
| Target Organs【靶器官】 | Kidney |
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
