Anti-Nestin Antibody, clone rat-401, Alexa Fluor^® 488 conjugate

32160702

NA.41

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Embryonic rat spinal cord

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.
Research Category
Stem Cell Research

Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Protein G Purified

Control
Mouse cortical neural stem cells (SCR029)

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ALEXA FLUOR is a registered trademark of Life Technologies

100

mouse

ALEXA FLUOR^® 488

purified immunoglobulin

primary antibodies

rat-401, monoclonal

mouse, rat

human

immunocytochemistry: suitable

IgG1

NP_037119

P21263

wet ice

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2