Anti-hnRNP F Antibody, clone 3H4

32160702

NA.41

The hnRNP F protein is member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. There are more than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) associated with pre-mRNAs in the nucleus of mammalian cells. These different hnRNPs display a range of affinities for a variety of ribonucleotidehomopolymers and ssDNA sequences. In constrast, hnRNP F protein as well as hnRNP H (a highly related protein) appear to bind only to poly(rG). These unique RNA-binding properties, as well as the amino acid sequence and other data showing a distinct intranuclear localization of hnRNP F and hnRNP H suggest they are unique from other hnRNP and play an important role in the processing of RNAs containing guanosine-rich sequences.

This antibody recognizes hnRNP F.

Recombinant protein corresponding to human hnRNP F.
Epitope: Unknown

Use Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory for IP. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)

Immunofluorescence Analysis:
A representative lot was used by an independent laboratory in IF. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected hnRNP F on 10 µg of K562 cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
K562 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

3H4, monoclonal

human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_001091674

P52597

wet ice

Calculated: 46 kDa Observed: ~53 kDa

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1