Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal

32160702

NA.41

Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

Broad species cross-reactivity expected based on sequence homology.
phospho-c-Src (Tyr416)

KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src

Research Sub Category
Cytoskeletal Signaling
Western Blot Analysis:
A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).

Immunoprecipitation:
A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).

Research Category
Signaling
Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB.

Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186)

Replaces: 05-857

Cultured supernantant in 0.05% sodium azide

Control
RIPA cell lysate

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

culture supernatant

primary antibodies

2N8, monoclonal

mouse, vertebrates

Upstate^®

western blot: suitable

IgG

NM_005417

P12931

dry ice

human ... SRC(6714)

60kDa

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

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12 - Non Combustible Liquids

WGK 1