Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12

32160702

NA.41

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Ser-7 is phosphorylated before initiation of transcription at promoter regions.

This antibody recognizes RNA polymerase II subunit B1 at the CTD when phosphorylated at Ser7.

Epitope: Ser7
Ovalbumin-conjugated linear peptide corrresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser7.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)
Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function
Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 (phospho-CTD Ser-7) has been validated in WB, ELISA.

Evaluated by Western Blot in γ-PPase untreated and treated NIH/3T3 cell lysates.

Western Blot Analysis: 0.25 µg/ml of this antibody detected RNA polymerase II CTD on 10 µg of γ-PPase untreated and treated NIH/3T3 cell lysates.

Format: Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

Control
γ-protein phosphatase (γ-Ppase) untreated and treated NIH/3T3 cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

rat

purified immunoglobulin

primary antibodies

4E12, monoclonal

mouse

human (based on 100% sequence homology)

ChIP: suitable
ELISA: suitable
western blot: suitable

IgG1κ

NP_000928

P24928

wet ice

~ 220 kDa

Stable for 1 year at 2-8°C from date of receipt.

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