Anti-HIRA Antibody, clone WC119

32160702

NA.41

HIRA (TUP1 like enhancer of split protein 1) is a protein localized primarily in the nucleus. It functions as a histone chaparone to preferentially place histone H3.3 on nucleosome. It is broadly expressed in a variety of tissues and is expressed during embryogenesis. Data suggests HIRA plays a part in mechanisms of transcriptional regulation similar to that played by yeast HIR1 and HIR2 together. The HIRA protein has been shown to interact with HIRIP3, HIRIP5, histones H2B and H4, and PAX3. HIRA may play a part in the etiology of the DiGeorge syndrome (DGS), a developmental disorder. The clinical features of this disease include absence or hypoplasia of the thymus and parathyroid glands, cardiovascular malformations, facial dysplasia, a cleft palate and mental retardation.

This antibody recognizes HIRA.

Epitope: Unknown
GST-tagged recombinant protein corresponding to human HIRA.

Anti-HIRA Antibody, clone WC119 is a Mouse Monoclonal Antibody for detection of HIRA also known as HIR (histone cell cycle regulation defective) homolog & has been validated in WB & IP.
Research Sub Category
Chromatin Biology

Chaperones
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Hall, C., et al. (2001). Molecular and Cellular Biology. 21(5):1854-1865.)
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 0.44 µg/ml of this antibody detected HIRA on 10 µg of NIH/3T3 cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
NIH/3T3 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

WC119, monoclonal

mouse

human (based on 100% sequence homology)

immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_003316.3

P54198

wet ice

human ... HIRA(7290)

~ 112 kDa

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 1