Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39

32160702

NA.41

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).

Predicted to cross-react with many species based on sequence homology.
Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.

Research Sub Category
MAP Kinases
Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex microspheres. (Figure B).

Research Category
Signaling

Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells

Replaces: 05-797

Format: Purified
Cultured supernantant in 0.05% sodium azide

Control
RIPA cell lysate from NGF stimulated PC-12 cells

Concentration: Variable

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

culture supernatant

primary antibodies

Aw39, monoclonal

human, rat

Upstate^®

multiplexing: suitable
western blot: suitable

IgG

NM_002745
NM_002746

P28482

dry ice

MAPK1 (~44 kDa) and MAPK2 (~42 kDa).

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1