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Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal
| 产品编号： | 4082612 |
| 规格： | culture supernatant, clone SA19, from rabbit |
| 包装规格： | 100 μl |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal
Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT. |
| Specificity【特异性】 | Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa. Predicted to cross-react with mouse and rat. |
| Immunogen【免疫原】 | KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1. Epitope: pThr850(Ch) or pThr853(H) |
| Application【应用】 | Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB. |
| Quality【质量】 | Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338). |
| Linkage【联系】 | Replaces: 05-773 |
| Analysis Note【分析说明】 | Control HEK293 cell lysate phosphorylated by ROKα/ROCKII. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | culture supernatant |
| antibody product type | primary antibodies |
| clone【克隆】 | SA19, monoclonal |
| species reactivity | human, rat, chicken, mouse |
| technique(s) | western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NP_002471.1 |
| UniProt accession no.【UniProt登记号】 | O14974 |
| shipped in【运输】 | dry ice |
产品说明
| Target description【目标描述】 | Doublet at ~130 kDa |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
