Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal

32160702

NA.41

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Predicted to cross-react with X. laevis based on sequence homology.
Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.

KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2

Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.

Routinely evaluated by immunoblot analysis.

Replaces: 05-953

Control
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

culture supernatant

primary antibodies

A5S, monoclonal

mouse, human, zebrafish, canine

Upstate^®

western blot: suitable

IgG

NM_001003652.2
NM_005901.4

Q15796

dry ice

human ... SMAD2(4087)

55-60 kDa

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10 - Combustible liquids

WGK 1