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GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus
| 产品编号： | 4082635 |
| 规格： | recombinant, expressed in E. coli, ≥70% (SDS-PAGE) |
| 包装规格： | 4 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus
E1A
基本信息
| NACRES | NA.26 |
| Biochem/physiol Actions【生化/生理作用】 | The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1. |
| Physical form【外形】 | Clear and colorless frozen liquid solution |
| Preparation Note【制备说明】 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
产品性质
| biological source【生物来源】 | Saccharomyces cerevisiae canine |
| recombinant【重组】 | expressed in E. coli |
| Assay【测定】 | ≥70% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| mol wt【分子量】 | ~33 kDa |
| packaging【包装】 | pkg of 4 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | 100 μg/mL |
| technique(s) | electrophoretic mobility shift assay: suitable |
| color【颜色】 | colorless to clear |
| NCBI accession no.【NCBI登记号】 | J04368 NM_001184062 |
| UniProt accession no.【UniProt登记号】 | P04386 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | Saccharomyces cerevisiae ... GAL4(855828) |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
