Anti-γ-Actin Antibody, clone 6J13 ZooMAb^® Rabbit Monoclonal

rabbit

200

expressed in HEK 293 cells

unconjugated

purified antibody

primary antibodies

6J13, monoclonal
recombinant monoclonal

ZooMAb^® learn more

lyophilized

calculated mol wt ~ 41.79 kDa
observed mol wt ~ 42 kDa

rat, mouse, human

bovine

antibody small pack of 25 μL

recombinant expression
Learn more about Antibody Enhanced Validation

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

IgG

ambient

2-8℃

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Clone 6J13 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects g-Actin. It targets an epitope within 13 amino acids from the N-terminal.

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human g-Actin.

Anti-g-Actin, clone 6J13 ZooMAb^®, Cat. No. ZRB1166, is a recombinant Rabbit monoclonal antibody that specifically targets g-Actin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected y-Actin in HepG2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected y-Actin in HeLa, C6, and mouse kidney tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected g-Actin in human small intestine tissue sections.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected g-Actin in one million HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected g-Actin in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Actin, cytoplasmic 2 (UniProt: P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG) gene (Gene ID: 71) in human. Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. -actin is a cytoplasmic actin that is highly conserved in higher animals and is predominantly expressed in non-muscle cells where it controls cell structure and motility. Unlike β-actin that is enriched at the leading edge of migrating cells, -actin is more uniformly distributed throughout the cell. -actin knockout is reported to cause developmental delays, reduced postnatal and adult survival, and progressive myopathy. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Belyantseva, EA., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(24); 9703-9708).

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1