广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-HNF-4alpha Antibody, clone 4C19 ZooMAb® Rabbit Monoclonal
产品搜索:

Anti-HNF-4alpha Antibody, clone 4C19 ZooMAb® Rabbit Monoclonal

产品编号:4083087
规格:recombinant, expressed in HEK 293 cells
包装规格:25 μL,4 X 25 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
408308725 μL2350
40830874 X 25 μL4400
产品别名

Anti-HNF-4alpha Antibody, clone 4C19 ZooMAb® Rabbit Monoclonal

Transcription factor 14

Hepatocyte nuclear factor 4-alpha

TCF-14

Nuclear receptor subfamily 2 group A member 1

Transcription factor HNF-4

产品性质
biological source【生物来源】
rabbit (recombinant)
recombinant【重组】
expressed in HEK 293 cells
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
4C19, monoclonal
recombinant monoclonal
product line【产品线】
ZooMAb® learn more
form【形式】
lyophilized
mol wt【分子量】
calculated mol wt 52.79 kDa
observed mol wt 53 kDa
species reactivity
rat, human, mouse
packaging【包装】
antibody small pack of 25 μL
greener alternative product characteristics【环保替代产品特性】
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation【增强验证】
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype【同位素/亚型】
IgG
greener alternative category【环保替代产品分类】
Aligned
shipped in【运输】
ambient
storage temp.【储存温度】
2-8℃
基本信息
General description【一般描述】
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.
Specificity【特异性】
Clone 4C19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detect Hepatocyte nuclear factor 4-alpha. It targets an epitope within 16 amino acids from the C-terminal region.
Immunogen【免疫原】
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Hepatocyte nuclear factor 4-alpha (HNF-4-alpha).
Application【应用】
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HNF-4-alpha in mouse liver nuclei.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected HNF-4-alpha in human liver, rat liver, and human colon tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected HNF-4-alpha in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound HNF-4-alpha peptide with a KD of 4.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-HNF-4, clone 4C19 ZooMAb, Cat. No. ZRB1457, is a recombinant Rabbit monoclonal antibody that specifically targets HNF-4 , and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Physical form【外形】
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution【重悬】
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Legal Information【法律信息】
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
产品说明
Target description【目标描述】
Hepatocyte nuclear factor 4-alpha (UniProt: P41235; also known as HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4) is encoded by the HNF4A (also known as HNF4, NR2A1, TCF14) gene (Gene ID: 3172) in human. HNF-4 is a transcriptional regulator that controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. It activates the transcription of CYP2C38 and represses the CLOCK-ARNTL/BMAL1 transcriptional activity. It is reported to be essential for circadian rhythm maintenance and period regulation in the liver and colon cells. It can undergo phosphorylation on tyrosine residues and that is shown to be important for its DNA-binding activity. However, phosphorylation at serine 313 by AMPK can reduce its ability to form homodimers and bind DNA. Mutations in HNF4A gene are known to cause type II diabetes. Changes in the HNF4A gene can reduce the amount of insulin that is produced by the pancreas. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Storage and Stability【储存及稳定性】
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
11 - Combustible Solids
WGK
WGK 1
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0085