(±)-Blebbistatin-CAS 674289-55-5-Calbiochem

C₁₈H₁₆N₂O₂

292.33

NA.77

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.
A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of nonmuscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤ 100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly. A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203389) in 90% DMSO is also available.

Cell permeable: yes
Primary Target
ATPase
Product does not compete with ATP.
Target IC₅₀: <100 µM inhibiting the ATPase and gliding motility of human platelets
Reversible: yes

Following reconstitution in 90% DMSO, aliquot and freeze (-20°C). Stock solutions in 90% DMSO are stable for up to 1 month at -20°C. Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥97% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

yellow

methanol: 1.5 mg/mL
100% DMSO: 100 mg/mL
90% DMSO: 75 mg/mL

wet ice

−20℃

5 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 2