Nitrocefin-CAS 41906-86-9-Calbiochem

C₂₁H₁₆N₄O₈S₂

516.50

A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow (λ_max = 390 nm at pH 7.0) to red (λ_max = 486 nm at pH 7.0) as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria. Also useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. Has been used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.

Toxicity: Flammable (J)

Preparing a Nitrocefin (500 µg/ml) Solution

• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.

• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.

• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.

• Nitrocefin, particularly in solution, is very sensitive to light.

Following reconstitution, aliquot and freeze (-20°C). Stock solution may be stored at -20°C for up to 2 weeks.

Guay, R., et al. 1980. IRCS Med. Science 8, 209.
King, A., et al. 1980. Antimicrob. Agents Chemother. 17, 165.
Matthew, M., et al. 1975. J. Gen. Microbiol. 88, 169.
O’Callaghan, C.H., et al. 1972. Antimicrob. Agents Chemother. 1, 283.
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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

200

≥95% (UV)

solid

Calbiochem^®

OK to freeze
protect from light

orange-yellow

ambient

−20℃

1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1

LHNIIDJCEODSHA-OQRUQETBSA-N

5 mg in Glass bottle
Packaged under inert gas

GHS05,GHS07

Danger

H315 - H317 - H318

P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

11 - Combustible Solids

WGK 3