Anti-E2F-1 Antibody, clones KH20 and KH95

32160702

NA.41

E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.

Recognizes E2F-1.

Epitope: The epitope for KH20 has been mapped to amino acids 1-89; KH95 to amino acids 342-386.
GST fusion protein corresponding to full-length human E2F-1.

Research Sub Category
Transcription Factors
Anti-E2F-1 Antibody, clones KH20 & KH95 is a high quality Mouse Monoclonal Antibody for the detection of E2F-1 & has been validated in IP & WB.
Research Category
Epigenetics & Nuclear Function

Immunoblot Analysis: 0.5-2μg/ml of this antibody detected E2F-1 in a HeLa nuclear extract.
Immunoprecipitation: 4μg of this antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Gel Shift: The KH20 clone has been shown to gel shift the E2F-1/DP-1/DNA complex (Helin, 1993; Wu, 2000).

Format: Purified
Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide.
Protein G Purified

Control
Positive Control Included: HeLa Nuclear Extract (12-309)

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

KH20, monoclonal
KH95, monoclonal

Xenopus, human

Upstate^®

immunoprecipitation (IP): suitable
western blot: suitable

IgG

NM_005225

Q01094

dry ice

55 kDa

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1