Anti-hnRNP U Antibody, clone 3G6

32160702

NA.41

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. However, its role in the regulation of gene expression is as yet poorly understood. Proteins of the heterogeneous nuclear ribonucleoparticles (hnRNP) family form a structurally diverse group of RNA binding proteins implicated in various functions. Recently, hnRNP proteins have been shown to hinder communication between factors bound to different splice sites. Conversely, several reports have described a positive role for some hnRNP proteins in pre-mRNA splicing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements.

This antibody recognizes hnRNP U.

Recombinant protein corresponding to human hnRNP U.
Epitope: Unknown

Immunoprecipitation
Reported by external laboratory using a represenative lot of this antibody
Immunocytochemistry
Reported by external laboratory using a represenative lot of this antibody
ELISA
Reported by external laboratory using a represenative lot of this antibody

Use Anti-hnRNP U Antibody, clone 3G6 (mouse monoclonal antibody) validated in WB, IP, ICC, ELISA to detect hnRNP U also known as heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Apoptosis - Additional

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.01 µg/ml of this antibody detected hnRNP U on 10 µg of K562 cell lysate.

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
K562 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

3G6, monoclonal

human

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG2bκ

NP_004492.2

Q00839

wet ice

Calculated molecular weight is 90 kDa However protein is usually observed at ~120 kDa (Dreyfuss G, Kim VN, Kataoka N. Nat Rev Mol Cell Biol. 2002 Mar;3(3):195-205.)

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1