广州伟伯科技有限公司
您的位置:首页 > 产品中心 > Anti-phospho-CREB (Ser133) Antibody, clone 634-2
产品搜索:

Anti-phospho-CREB (Ser133) Antibody, clone 634-2

产品编号:4084705
规格:clone 634-2, Upstate®, from mouse
包装规格:200 μg
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4084705200 μg5520
产品别名

Anti-phospho-CREB (Ser133) Antibody, clone 634-2

active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
CREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.
Specificity【特异性】
Phospho-CREB (Ser133).
Broad species cross-reactivity expected, based on sequence homology.
Immunogen【免疫原】
Epitope: Ser133
A proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
Application【应用】
Research Sub Category
Transcription Factors
Beadlyte Specificity Assay:
1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence

Immunofluorescence / Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Use Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
Quality【质量】
Routinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells.

Western Blot Analysis:
A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A).
Physical form【外形】
Purified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC.
Format: Purified
Protein G Purified
Analysis Note【分析说明】
Control
Human breast carcinoma, forskolin- and FGF-treated SK-N-MC cell extracts.
Other Notes【其他说明】
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information【法律信息】
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
Quality Level【质量水平】
100
biological source【生物来源】
mouse
antibody form【抗体形式】
purified antibody
antibody product type
primary antibodies
clone【克隆】
634-2, monoclonal
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
isotype【同位素/亚型】
IgG1κ
NCBI accession no.【NCBI登记号】
NM_134442
UniProt accession no.【UniProt登记号】
P16220
shipped in【运输】
dry ice
产品说明
Target description【目标描述】
43 kDa
Storage and Stability【储存及稳定性】
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
广州伟伯科技有限公司 版权所有 CopyRight ©2006-2024, All Rights Reserved
工信部备案号:粤ICP备08114744号   Page Run Time: 0.0096