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Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
产品别名
Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
insulin receptor substrate 1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop. |
Specificity【特异性】 | Predicted to cross-react with many other species based on 100% sequence homology with immunogen. This antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human). |
Immunogen【免疫原】 | Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1. |
Application【应用】 | This Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 is validated for use in WB, IP, IC, IF for the detection of phospho-IRS1 (Ser307 mouse/ Ser312 human). |
Quality【质量】 | Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate. Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate. |
Linkage【联系】 | Replaces: 07-247 |
Physical form【外形】 | Protein G Purified Format: Purified |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 24.6.2, monoclonal |
species reactivity | pig, human, monkey, canine, bovine, rat, mouse |
technique(s) | immunocytochemistry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_005535.1 |
UniProt accession no.【UniProt登记号】 | P35568 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | ~185 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |