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Anti-U1-70K Antibody, clone 9C4.1
产品别名
Anti-U1-70K Antibody, clone 9C4.1
small nuclear ribonucleoprotein 70kDa (U1), U1 small nuclear ribonucleoprotein 70 kDa, small nuclear ribonucleoprotein 70kDa (RNP antigen), U1 snRNP 70 kDa
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA. |
Specificity【特异性】 | This antibody recognizes U1-70K at the C-terminal domain. |
Immunogen【免疫原】 | Epitope: C-terminal domain Recombinant protein corresponding to C-terminal domain of human U1-70K. |
Application【应用】 | Use Anti-U1-70K Antibody, clone 9C4.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1). Immunocytochemistry Analysis: A 1:500 dilution of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells. Research Category Epigenetics & Nuclear Function Research Sub Category RNA Metabolism & Binding Proteins |
Quality【质量】 | Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HeLa cell lysate. |
Physical form【外形】 | Format: Purified Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Protein G Purified |
Analysis Note【分析说明】 | Control HeLa cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 9C4.1, monoclonal |
species reactivity | mouse, human, rat |
technique(s) | immunocytochemistry: suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_003080.2 |
UniProt accession no.【UniProt登记号】 | P08621 |
shipped in【运输】 | wet ice |
Gene Information | human ... SNRNP70(6625) |
产品说明
Target description【目标描述】 | Calculated 52 kDa Observed ~57 kDa |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |