Anti-SMN Antibody, clone 2B1

32160702

NA.41

The survival of motor neurons (SMN) protein is essential for the biogenesis of small nuclear RNA (snRNA)-ribonucleoproteins (snRNPs), the major components of the pre-mRNA splicing machinery. Though it is ubiquitously expressed, SMN deficiency causes the motor neuron degenerative disease spinal muscular atrophy (SMA). SMN deficiency has unexpected cell type-specific effects on the repertoire of snRNAs and mRNAs. It alters the stoichiometry of snRNAs and causes widespread pre-mRNA splicing defects in numerous transcripts of diverse genes, preferentially those containing a large number of introns, in SMN-deficient mouse tissues. The SMN complex plays a role in RNA metabolism and in splicing regulation.

This antibody recognizes SMN.

His-tagged recombinant protein corresponding to human SMN.
Epitope: Unknown

Anti-SMN Antibody, clone 2B1 is a Mouse Monoclonal Antibody for detection of SMN also known as Spinal muscular atrophy (Werdnig-Hoffmann disease Kugelberg-Welander disease) & has been validated in WB, ICC & IP.
Research Sub Category
RNA Metabolism & Binding Proteins

RNA Metabolism & Binding Proteins
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SMN in HeLa cells.

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected SMN on 10 µg of HeLa cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

2B1, monoclonal

human

Xenopus, mouse

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_000335.1

Q16637

wet ice

human ... SMA4(11039)

35 kDa was observed; however, the calculated molecular weight is 31.849 kDa

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 1