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Anti-TCF-4 Antibody, clone 6H5-3
产品别名
Anti-TCF-4 Antibody, clone 6H5-3
HMG box transcription factor 4, Transcription factor 7-like 2, T-Cell factor 4, T-cell-specific, HMG box, T-Cell-specific transcription factor 4, Transcription factor 7-like 2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes. |
| Specificity【特异性】 | This antibody recognizes TCF-4 |
| Immunogen【免疫原】 | Amino acids 31-331 of human TCF-4. |
| Application【应用】 | Immunocytochemistry/Immunohistochemistry: A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003). Immunoprecipitation: A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001). Supershift Assay: A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003). Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2. |
| Quality【质量】 | Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4. Western Blot Analysis: 0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174). |
| Linkage【联系】 | Replaces: 04-1080 |
| Physical form【外形】 | Protein G Chromatography Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Format: Purified |
| Analysis Note【分析说明】 | Control DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells. |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Legal Information【法律信息】 | GenBank is a registered trademark of United States Department of Health and Human Services UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | 6H5-3, monoclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Upstate® |
| technique(s) | electrophoretic mobility shift assay: suitable immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG2a |
| GenBank® accession no. | CAB97213.1 |
| UniProt accession no.【UniProt登记号】 | Q9NQB0 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... TCF7L2(6934) |
产品说明
| Target description【目标描述】 | Mr 66 kDa |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
