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SV40 T-antigen from simian virus
| 产品编号： | 4085393 |
| 规格： | recombinant, expressed in insect cells, ≥80% (SDS-PAGE) |
| 包装规格： | 5 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品性质
| biological source【生物来源】 | simian virus |
| recombinant【重组】 | expressed in insect cells |
| Assay【测定】 | ≥80% (SDS-PAGE) |
| form【形式】 | frozen liquid |
| mol wt【分子量】 | ~83.3 kDa |
| packaging【包装】 | pkg of 5 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| concentration【浓度】 | 400 μg/mL |
| color【颜色】 | clear colorless |
| NCBI accession no.【NCBI登记号】 | AAG39214.1 |
| UniProt accession no.【UniProt登记号】 | P03070 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −70℃ |
| Gene Information | simian virus ... SV40gp6(1489531) |
基本信息
| Biochem/physiol Actions【生化/生理作用】 | The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively. The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them. |
| Physical form【外形】 | Clear and colorless frozen liquid solution |
| Preparation Note【制备说明】 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
