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基本信息
| NACRES | NA.51 |
| General description【一般描述】 | The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA |
| Application【应用】 | Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology |
| Features and Benefits【特点和优势】 | HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion. |
| Physical form【外形】 | Dry |
| Preparation Note【制备说明】 | Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less. |
| Legal Information【法律信息】 | CRISPR Use License Agreement SygRNA is a registered trademark of sigma-aldrich Co. LLC |
产品性质
| packaging【包装】 | pkg of 1 vial |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
