Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1

32160702

NA.41

Basic fibroblast growth factor, also known as bFGF or FGF2, is a member of the fibroblast growth factor family.
In normal tissue, basic fibroblast growth factor is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels, a process known as angiogenesis.
Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.

This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.
This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.

Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.
Epitope: a.a. 5-24

Research Sub Category
Growth Factors & Receptors
Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 is an antibody against FGF-2/basic FGF (neutralizing) for use in NEUT, RIA.
Research Category
Signaling

Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.

Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.

Format: Purified
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.

Control
Fetal liver or kidney tissue.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified immunoglobulin

primary antibodies

bFM-1, monoclonal

bovine, human, rat, mouse

Upstate^®

neutralization: suitable
radioimmunoassay: suitable

IgG1κ

not suitable for Western blot

NM_002006.3

P09038

dry ice

17.5 kDa

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1