您的位置：首页 > 产品中心 > FLYA13
产品别名
FLYA13
FLYA-13
FLYA
产品性质
| biological source【生物来源】 | human fibrosarcoma |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Epithelial-like |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
基本信息
| Cell Line Origin【细胞系来源】 | Human fibrosarcoma (retroviral packaging cells) |
| Cell Line Description【细胞系描述】 | FLYA13 has been developed together with FLYRD18 (Sigma catalog number 95091902) for packaging of retroviruses, it is especially useful for in vivo gene therapy. Both cell lines have been derived from HT1080 cells (Sigma catalog number 85111505). FLYA13 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of amphotropic murine leukaemia virus. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000 i.u./ml. Addition of 4μg/ml blasticidin S and 10μg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line. |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X,Y, CSF1PO: 12 D13S317: 12,14 D16S539: 9,12 D5S818: 11,13 D7S820: 9,10 THO1: 6 TPOX: 8 vWA: 14,19 |
| Culture Medium【培养基】 | DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:20, i.e., seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品说明
| Disclaimer【免责声明】 | This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form. |
