抗细胞周期蛋白B1抗体，克隆GNS3(8A5D12)

32160702

NA.41

G2/mitotic-specific cyclin-B1 (UniProt: P14635; also known as Cyclin B1) is encoded by the CCNB1 (also known as CCNB) gene (Gene ID: 891) in human. Cyclins are the regulatory subunits of the cell cycle-dependent kinases (CDKs) that are responsible for the phosphorylation of several cellular targets. Cyclins contain the nuclear localization sequence (NLS) that help move CDKs into the nucleus. They also contain PEST (Pro, Glu, Ser, and Thr) sequences that target them for degradation by the ubiquitin-proteasomal pathway. Once the CDKs have completed their role, they undergo a rapid programmed proteolysis via ubiquitin-mediated delivery to the proteasome complex. Cyclin B1, a regulatory protein involved in mitosis, complexes with CDK1 to form the maturation-promoting factor (MPF). It is shown to be essential for the control of the cell cycle at the G2/M (mitosis) transition. It accumulates steadily during G2 phase and is abruptly destroyed at mitosis. Hence, the cyclin B1-CDK1 complex is considered to be a key regulator for mitotic entry. This complex phosphorylates a number of proteins prior to mitotic entry. Although five serine phosphorylation sites are described for cyclin B1, (Ser 116, 126, 128, 133, and 147), serine 133 phosphorylation by PLK1 regulates the entry of Cyclin B1-CDK1 complex into the nucleus during prophase. At the end of mitosis, cyclin B1 is rapidly removed by a ubiquitin ligase (anaphase-promoting complex/cyclosome) loaded with the targeting subunit CDC20. Activated cyclin B1-CDK1 complex is reported to catalyze its own destruction by stimulating the activity of APC. (Ref.: Van Zon, W., et al. (2010). J. Cell. Biol. 190(4); 587-602; Yuan, J., et al. (2004). Oncogene 23(34); 5843-5852).

该抗体对人类细胞周期蛋白B1(分子量58 kDa)具有特异性。不会与细胞周期蛋白A或细胞周期蛋白D发生交叉反应。
除了人以外，还观察到其与小鼠的弱种属交叉反应性。

Immunoprecipitation:
2 μg of a previous lot immunoprecipitated human cyclin B1 and cdc2 kinase from 500 μg of A431 RIPA lysate as determined by a subsequent immunoblot of the immunoprecipitate using anti-cdk1/cdc2 (PSTAIR), (Catalog # 06-923).
Western Blotting Analysis: 0.1 mg/mL of this antibody detected Cyclin B1 in A431 cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:250 and 1:50 dilutions of this antibody detected Cyclin B1 in Human tonsil and Human bone marrow tissue sections, respectively.

已通过蛋白质印迹分析对来自人A431癌细胞的RIPA裂解物进行了常规评估。

蛋白质印迹分析：
0.5-2 μg/mL该批次产品在人A431癌细胞的RIPA裂解物中检测到细胞周期蛋白B1。

替代品：04-220

Protein G Chromatography
蛋白G纯化的小鼠IgG，保存在含有0.1 M Tris-甘氨酸(pH 7.4)、0.15 M NaCl、0.05％叠氮化钠的缓冲液中。冻存于 -20°C。
形式：纯化

对照
A431细胞
包含的阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。加入 2.5 μL 2-巯基乙醇/100 μL 裂解液，煮沸 5 分钟以减少制备量。每个泳道上样 20 μg 还原裂解液以制备小凝胶。

浓度：请参考批次特异性浓缩物的分析证书。

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

purified antibody

primary antibodies

GNS3 (8A5D12), monoclonal

mouse, human

antibody small pack of 25 μg

Upstate^®

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG

NM_031966

P14635

dry ice

58kda

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