您的位置：首页 > 产品中心 > Anti-CEP135
产品别名
Anti-CEP135
Centrosomal protoein of 135 kDa, Centrosomal protein 4
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Centrosomal protein of 135 kDa (UniProt: Q66GS9; also known as Cep135, Centrosomal protein 4) is encoded by the CEP135 (also known as CEP4, KIAA0635) gene (Gene ID: 9662) in human. Cep135 is a centrosomal protein that is involved in centriole biogenesis. Human Cep135 is reported to contain two-stranded coiled-coil domains that are critical for microtubule binding. Cep135 acts as a scaffolding protein during early centriole biogenesis and is required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. It also plays a role in centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Mutations in CEP135 gene are associated with autosomal recessive primary microcephaly-8, a disease characterized by intellectual disability, markedly reduced brain weight, and small cerebral cortex. |
| Specificity【特异性】 | This polyclonal antibody detects centrosomal protein of 135 kDa in human cells. It targets an epitope within 492 amino acids from the C-terminal half. |
| Immunogen【免疫原】 | His-tagged recombinant fragment corresponding to 492 amino acids from the C-termnal region of human Cep135. |
| Application【应用】 | Anti-CEP135, Cat. No. ABE1857, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 135 kDa and has been tested in Electron Microscopy, Immunocytochemistry, and Western Blotting. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CEP135 in synchronized U2OS cells. Electron Microscopy Analysis: A representative lot detected CEP135 in Electron Microscopy applications (Kleylein-Sohn, J., et. al. (2007). Dev Cell. 13(2):190-202). Immunocytochemistry Analysis: A representative lot detected CEP135 in Immunocytochemistry applications (Kleylein-Sohn, J., et. al. (2007). Dev Cell. 13(2):190-202; Guderian, G., et. al. (2010). J Cell Sci. 123(Pt 13):2163-9; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76). |
| Quality【质量】 | Evaluated by Western Blotting in U2OS cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected CEP135 in 10 µg of U2OS cell lysate. |
| Physical form【外形】 | Protein A purified Format: Purified |
| Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | human |
| packaging【包装】 | antibody small pack of 25 μg |
| technique(s) | electron microscopy: suitable immunocytochemistry: suitable western blot: suitable |
| isotype【同位素/亚型】 | IgG |
| NCBI accession no.【NCBI登记号】 | NP_079285 |
| UniProt accession no.【UniProt登记号】 | Q66GS9 |
| shipped in【运输】 | ambient |
| Gene Information | human ... CEP135(9662) |
产品说明
| Target description【目标描述】 | ~150 kDa observed; 133.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| 【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
