Anti-Histone Macro H2A.1 Antibody

32160702

NA.41

Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.

This antibody recognizes Histone MacroH2A.1 at the macro domain.

GST-tagged recombinant protein corresponding to mcro domain of mouse Histone MacroH2A.1.
Epitope: Macro domain

Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone MacroH2A.1 in NIH/3T3, A431, and HeLa cells.
Anti-Histone Macro H2A.1 Antibody is a Rabbit Polyclonal Antibody for detection of Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2 & has been validated in WB, ICC.

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract.

Replaces: 07-219

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa acid extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

mouse, human

immunocytochemistry: suitable
western blot: suitable

NP_001152985.1

Q9QZQ8

wet ice

human ... H2AFJ(55766)

~ 40 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1