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产品别名
Anti-WAC Antibody, CT
WW domain-containing adapter protein with coiled-coil, WAC
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | WAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at ′Lys-120′ (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the C-terminus of the WAC protein. |
Immunogen【免疫原】 | Recombinant protein corresponding to human WAC. |
Application【应用】 | Anti-WAC, C-terminus detects levels of WAC proteins & has been published & validated for use in wb, IP & ChIP. Research Category Epigenetics & Nuclear Function Research Sub Category Chromatin Biology Western Blotting Analysis: 1 µg/mL of this antibody from a representative lot detected WAC, C-terminus in 10 µg of HEK293 cell lysate. Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.). Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.). Westerm Blotting Analysis: A representative lot from an independent laboratory detected WAC in 293T cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in 293T cell lysates transfected with siWAC (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.). |
Quality【质量】 | Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, C-terminus in 10 µg of K562 cell lysate. |
Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Format: Purified Affinity purified |
Analysis Note【分析说明】 | Control K562 cell lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | affinity isolated antibody |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | human |
technique(s) | ChIP: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NP_057712 |
UniProt accession no.【UniProt登记号】 | Q9BTA9 |
shipped in【运输】 | wet ice |
Gene Information | human ... WAC(51322) |
产品说明
Target description【目标描述】 | ~80 kDa observed. An uncharacterized band may be observed at ~95 kDa and ~120 kDa in some cell lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |