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Anti-CLIM-1/2 Antibody

产品编号:4087809
规格:serum, from rabbit
包装规格:100 μL
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4087809100 μL5350
产品别名

Anti-CLIM-1/2 Antibody

LIM domain-binding protein 2, LDB-2, Carboxyl-terminal LIM domain-binding protein 1, CLIM-1, LIM domain-binding factor CLIM1, CLIM-1/2

基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
LIM domain-binding protein 1 (UniProt P70662; CLIM-2, LDB-1) encoded by the murine Ldb2 gene (Clim2, Nli; Gene ID 16825) and LIM domain-binding protein 2 (UniProt O55203; CLIM-1, LDB-2, CLP-36) encoded by the murine Ldb2 gene (Clim1; Gene ID 16826) are LIM domain-interacting cofactors whose interaction with LIM homeodomain (LIM-HD) transcription factors are crucial for the development of specific types of interneurons and motor neurons during neural tube development. The ubiquitin ligase RLIM targets CLIM for proteasomal degradation, thereby inhibiting developmental LIM-HD activity.
Specificity【特异性】
Reacts with both CLIM1 and CLIM2. Expected to react with all spliced isoforms of CLIM1 (CLIM-1a, CLIM-1b, and isoform 3) & CLIM2 (Visvader-a, Tran-a, Tran-b).
Immunogen【免疫原】
Recombinant protein corresponding to mouse CLIM-1.
Application【应用】
Western Blotting Analysis: A representative lots detected endogenous CLIM1/2 in CHO and murine pituitary gonadotroph Alpha T3 cells, as well as bacterially expressed and in vitro tranlsated recombinant CLIM1/2 (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791; Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunocytochemistry Analysis: A representative lot revealed an inverse correlation between the expression level of endogenous CLIM1/2 and that of its negative regulator RLIM in HEK293, CHO, murine pituitary gonadotroph Alpha T3, and chicken ectodermal cells by dual fluorescent immunocytochemistry (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CLIM1/2 occupancy at the alphaGSU promoter in murine pituitary gonadotroph Alpha T3 cells (Ostendorff, H.P., et al. (2002). Nature. 416(6876):99-103).
Immunohistochemistry Analysis: A representative lot detected similar stage-dependent expression patterns between CLIM1/2 and the ubiquitin ligase RLIM in the developing neural tube during early mouse embryogenesis (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
Immunofluorescence Analysis: A representative lot revealed highly co-localized expressions of CLIM1/2 and RLIM on thoracic sections from E12.5 mouse embryos by dual fluorescent immunohistochemistry (Ostendorff, H.P., et al. (2006). Dev Dyn. 235(3):786-791).
This Anti-CLIM-1/2 Antibody is validated for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunofluorescence for the detection of CLIM-1/2.
Quality【质量】
Evaluated by Western Blotting in E16 embryonic mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected CLIM-1/2 in 10 µg of E16 embryonic mouse brain tissue lysate.
Other Notes【其他说明】
Unpurified
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
hamster, mouse, chicken, human
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_034828
UniProt accession no.【UniProt登记号】
O55203
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
~50/43 kDa observed. Uncharacterized band(s) may appear in some lysates.
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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