您的位置：首页 > 产品中心 > Anti-Smad4/DPC4 Antibody
Anti-Smad4/DPC4 Antibody
| 产品编号： | 4087896 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Smad4/DPC4 Antibody
Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | SMAD4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. SMAD4 is functionally distinct among the SMAD family, and is required for the assembly and transcriptional activation of diverse, SMAD-DNA complexes. SMAD4 appears to be not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses. SMAD4 is frequently inactivated in pancreatic, biliary and colorectal tumors. |
| Specificity【特异性】 | This antibody recognizes Smad4 at the SAD region. |
| Immunogen【免疫原】 | GST-tagged recombinant protein corresponding to human Smad4 at the SAD region. Epitope: SAD region |
| Application【应用】 | Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Smad4 in NIH/3T3, HeLa, and A431 cells. Research Sub Category Transcription Factors Use Anti-Smad4/DPC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Smad4/DPC4 also known as Mothers against decapentaplegic homolog 4, SMAD 4, Smad4, hSMAD4. Research Category Epigenetics & Nuclear Function |
| Quality【质量】 | Evaluated by Western Blot in HepG2 cell lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected Smad4 on 10 µg of HepG2 cell lysate. |
| Linkage【联系】 | Replaces: 04-1033 |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control HepG2 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, human, rat |
| species reactivity (predicted by homology) | chimpanzee (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_005350.1 |
| UniProt accession no.【UniProt登记号】 | Q13485 |
| shipped in【运输】 | wet ice |
| Gene Information | human ... SMAD4(4089) |
产品说明
| Target description【目标描述】 | ~68 kDa observed. |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
