Anti-Spastin Antibody

32160702

NA.41

Spastin is a distinct microtubule-severing protein. Autosomal dominant mutations in the SPG4 locus corresponding to spastin are the most common cause of hereditary spastic paraplegia (HSP), a neurodegenerative disease that afflicts the adult corticospinal tracts.

Recombinant protein corresponding to human Spastin with 4 isoforms.

Western Blotting Analysis: A representative lot from an independent laboratory detected Spastin in myc-Spastin transfected HeLa and MRC5 cell lysates (Connell, et al. (2009). Traffic. 10:42-56).
Detect Spastin using this rabbit polyclonal antibody, Anti-Spastin Antibody validated for use in western blotting & ICC.

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Spastin in 10 µg of L6 cell lysate.

rabbit

100

serum

primary antibodies

polyclonal

human, rat, mouse

immunocytochemistry: suitable
western blot: suitable

NP_055761

Q9UBP0

wet ice

human ... SPAST(6683)

~ 68, 64, 58, 55 kDa observed. Uniprot describes 4 isoforms produced by alternative splicing at ~68 kDa, ~64 kDa, ~58 kDa, and ~55 kDa Spastin has shown in some western blots that four isoforms [full length (68 kD), exon 4-deleted full length (64 kD), short form (58-60 kD) and exon 4-deleted short form (55 kD)] are expressed in mammalian cells. Ths pAb detects bands corresponding to the size of these four isoforms in HeLa, MRC5 human lung fibroblast and NSC34 mouse lower motor neuron neuroblastoma fusion cell lines (Connell, et al. (2009). Traffic. 10:42-56).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1