Anti-APP A4 Antibody, a.a. 66-81 (NT), Prediluted, clone 22C11

32160702

NA.41

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta/A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins are currently under investigation Reacts with pre-A4.

The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Epitope: a.a. 66-81 (N-terminus), Prediluted
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Research Category
Neuroscience
Detect Alzheimer Precursor Protein A4 using this Anti-APP A4 Antibody, a.a. 66-81 (N-terminus), Prediluted, clone 22C11 validated for use in IH(P).
Research Sub Category
Neurodegenerative Diseases
Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select Detection Kits.

App has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No. IHCR1002-6.

Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

mouse

primary antibodies

22C11, monoclonal

pig, fish, rat, mouse, human, canine, monkey

Chemicon^®

immunohistochemistry: suitable (paraffin)

IgG1

NM_000484.2
NM_201413.1
NM_201414.1

P05067

wet ice

human ... APP(351)

Maintain at 2-8°C. Refer to vial for expiration dating.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GHS07

Warning

H317 - H412

P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313

Aquatic Chronic 3 - Skin Sens. 1

12 - Non Combustible Liquids

WGK 2