Anti-acetyl-Histone H4 Antibody

32160702

NA.41

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Recognizes acetylated histone H4.
Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
Other species not tested.

KLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.
Epitope: a.a. 2-19

Immunoprecipitation:
5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours.

Immunocytochemistry:
5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS.

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Anti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.

Routinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
Protein A chromatography
Format: Purified

Control
TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin

primary antibodies

polyclonal

Tetrahymena sp., human

Upstate^®

1 mg/mL

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG

NM_175054

P62805

dry ice

10 kDa

1 year at -20°C from date of shipment.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK 1