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Anti-Sonic Hedgehog Antibody
| 产品编号： | 4088129 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-Sonic Hedgehog Antibody
sonic hedgehog, sonic hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila)
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma. |
| Specificity【特异性】 | This antibody recognizes the N-terminus of Sonic Hedgehog. |
| Immunogen【免疫原】 | Epitope: N-terminus GST-tagged recombinant protein corresponding to the N-terminus of Sonic HedgeHog. |
| Application【应用】 | Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate. Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Sonic Hedgehog in colorectal carcinoma tissue. Anti-Sonic Hedgehog Antibody detects level of Sonic Hedgehog & has been published & validated for use in WB, IH. Research Category Signaling Research Sub Category Developmental Signaling |
| Quality【质量】 | Evaluated by Western Blot in human fetal skeletal muscle tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate. |
| Linkage【联系】 | Replaces: 04-971 |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control Human fetal skeletal muscle tissue lysate |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | mouse, human |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_000184 |
| UniProt accession no.【UniProt登记号】 | Q15465 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~ 45 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
