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Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody
| 产品编号： | 4088186 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody
Histone H1.4, Histone H1b
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues. |
| Specificity【特异性】 | Other homologies: Mouse and Rat (90% sequence homology). This antibody recognizes Histone H1 acetylated at Lys34 and phosphorylated at Ser36. |
| Immunogen【免疫原】 | Epitope: Acetylated Lys34 and phosphorylated Ser36 KLH-conjugated linear peptide corresponding to human Histone H1 acetylated at Lys34 and phosphorylated at Ser36. |
| Application【应用】 | Research Sub Category Histones Research Category Epigenetics & Nuclear Function Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with acetyl (Lys34) phospho (Ser36) Histone H1. Use Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody (Rabbit Polyclonal Antibody) validated in WB & DB to detect acetyl (Lys34) phospho (Ser36) Histone H1 also known as Histone H1b. |
| Quality【质量】 | Evaluated by Western Blot in calf thymus histone extract. Western Blot Analysis: A 1:37 dilution of this antibody detected Histone H1 on 10 µg of calf thymus histone extract. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control Calf thymus histone extract |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | bovine, human |
| species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology) |
| technique(s) | dot blot: suitable western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NP_005312 |
| UniProt accession no.【UniProt登记号】 | P10412 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~34 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
