Anti-Cdk1/Cdc2 (PSTAIR) Antibody

32160702

NA.41

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.

Cdk1/cdc2 kinase
Wide species cross-reactivity is expected.

Synthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species.

Fluorescent Immunocytochemistry Analysis:
A previous lot of this antibody was used in the confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor488), and DAPI (Blue, Nucleus). Positive nuclear staining.

Immunoprecipitation: 4 µg of a previous lot immunoprecipitated cdc2 from 500 µg of A431 RIPA lysate.

Anti-Cdk1/Cdc2 (PSTAIR) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Cdk1/Cdc2 (PSTAIR) & has been validated in ICC, IP & WB.

Evaluated by western blot on human A431, mouse 3T3 and/or rat L6 RIPA cell lysates.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected cdc2 from human A431, mouse 3T3 or rat L6 RIPA cell lysates.

Purified rabbit serum in Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Antigen Affinity Purified
Format: Purified

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

amphibian, mouse, human, rat

starfish, monkey, sheep, Drosophila, frog, canine, chicken

Upstate^®

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG

NM_001786

P06493

wet ice

~34 kDa

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1