Anti-Histone H3 Antibody

32160702

NA.41

Histone H3, also known as Histone H3.1t, H3/t, H3t, or H3/g, and encoded by the gene HIST3H3/H3Ft, is one of the core components of the nucleosome. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The octamer wraps approximately 147 bp of DNA. A classic nuclear marker, new expression is seen during S phase of the cell cycle. Histone H3 is often acetylated and or methylated multiple times. Changes in methylation or acetylation state of histone H3 together with similar modification on the sister histones is one key aspect of epigenetic gene expression and regulation.

This antibody recognizes Histone H3 at the N-terminus.
Broad species cross-reactivity is expected based on sequence similarity.

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3.
Epitope: N-terminus

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in HeLa cells.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Histone H3 from 500 µg of HeLa acid extract. Immunoprecipitated sample was then subjected to Western Blotting using 0.5 µg/mL of the same antibody.

Chromatin Immunoprecipitaton: A representative lot from an independent laboratory immunoprecipitated Histone H3 from plant chromatin (Ng, D. W., et al. (2006). Plant Cell. 18(1):119-132.).
Research Sub Category
Histones
Anti-Histone H3 Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 also known as histone 3-H3 & has been validated in ChIP, ICC & WB.
Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa acid extract.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Control
HeLa acid extract

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rat, mouse

Upstate^®

0.5 mg/mL

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NP_003484

Q16695

wet ice

~17 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1