Anti-phospho-IKK-epsilon (Ser172) Antibody

32160702

NA.41

Inhibitor of nuclear factor kappa-B kinase subunit epsilon (UniProt: Q14164; also known as EC: 2.7.11.10, I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, Inducible I kappa-B kinase, IKK-i) is encoded by the IKBKE (also known as IKKE, IKKI, KIAA0151) gene (Gene ID: 9641) in human. IKK-epsilon is a homodimeric serine/threonine kinase that is highly expressed in spleen, thymus, peripheral blood leukocytes, pancreas, and placenta. Its activity is induced by lipopolysaccharide and TNF-alpha. It plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B, and STAT signaling. IKK-epsilon can be either be auto-phosphorylated or phosphorylated by IKBKB/IKKB and phosphorylation at serine172 is enhanced by the interaction with ATP-dependent RNA helicase DDX3X. Its ATP-binding site resides in amino acids 15 to 23. IKK-epsilon is reported to phosphorylate inhibitors of NF-kappa-B leading to their ubiquitination and degradation. Following activation of viral RNA sensors, such as RIG-I-like receptors, it associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Two isoforms of IKK-epsilon have been described that are produced by alternative splicing.

This antibody recognizes IKK-epsilon phosphorylated at Ser172.

Epitope: Phosphorylated Ser172
Linear peptide corresponding to human IKKepsilon phosphorylated at Ser172.

This Anti-phospho-IKK-epsilon (Ser172) Antibody is validated for use in WB for the detection of phospho-IKK-epsilon (Ser172).
Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology

Evaluated by Western Blot in CD3+CD28 treated and untreated Jurkat cell lysates.

Western Blot Analysis: 1:1,000 dilution of this antibody detected IKK-epsilon on 10 µg of CD3+CD28 treated and untreated Jurkat cell lysates.

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 30% glycerol.
Affinity purified

Control
CD3+CD28 treated and untreated Jurkat cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, mouse

rat (based on 100% sequence homology)

western blot: suitable

NP_001180251

Q14164

wet ice

~90 kDa observed

Stable for 1 year at -20°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1