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Anti-ECT2 Antibody
| 产品编号： | 4088435 |
| 规格： | from rabbit, purified by affinity chromatography |
| 包装规格： | 100 μg |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-ECT2 Antibody
epithelial cell transforming sequence 2 oncogene, Epithelial cell-transforming sequence 2 oncogene, protein ECT2
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Epithelial cell-transforming sequence 2 oncogene (ECT2) is a guanine nucleotide exchange factor or GEF that belongs to the Dbl family of Rho GEFs. ECT2 is composed of 2 BRCT domains within the N-terminus, a DBL-homology domain, and a PH domain. Studies have shown ECT2 to localize to both the cell cortex and central spindles, and that it is a crucial part of cleavage furrow formation during cytokenises. Overexpression of cleaved ECT2, containing only the N-terminal domain, has been shown to cause multinucleation. As a GEF it has been shown to bind specifically to Rac proteins, RhoA, and RhoC. |
| Specificity【特异性】 | This antibody recognizes ECT2 at the C-terminus. |
| Immunogen【免疫原】 | KLH-conjugated linear peptide corresponding to human ECT2 at the C-terminus. Epitope: C-terminus |
| Application【应用】 | Anti-ECT2 Antibody is an antibody against ECT2 for use in WB & IC. Research Category Signaling Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected ECT2 in A549 cell lysate. Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells. (Der Channing, University of North Carolina at Chapel Hill, NC, channing_der@med.unc.edu) Research Sub Category G-proteins |
| Quality【质量】 | Evaluated by Western Blot in A549 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected ECT2 on 10 µg of A549 cell lysate. |
| Physical form【外形】 | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Affinity purified |
| Analysis Note【分析说明】 | Control A549 cell lysate |
| Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| purified by【纯化方式】 | affinity chromatography |
| species reactivity | pig, human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), opossum (based on 100% sequence homology), rabbit (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable western blot: suitable |
| UniProt accession no.【UniProt登记号】 | Q9H8V3 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | ~ 105 kDa observed |
| Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
