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Anti-GLUT-1 Antibody, CT
产品编号: | 4088443 |
规格: | from rabbit, purified by affinity chromatography |
包装规格: | 50 μg |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-GLUT-1 Antibody, CT
Glucose Transporter type 1, Solute carrier family 2, facilitated glucose transporter, member 1
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. GLUT2 is a facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. GLUT-4 is present in skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species. The expression of GLUT-4 is greatly influenced by insulin and other stimulators of the IP3 signaling pathway. Binding of insulin to the insulin receptor signals through PI3K and Akt to translocate GLUT-4 to the plasma membrane where it allows the uptake of glucose. |
Specificity【特异性】 | This rabbit polyclonal antibody detects GLUT-1 in human and mouse cells. It targets an epitope within 12 amino acids from the C-terminal half. |
Immunogen【免疫原】 | Epitope: C-Terminus Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV). |
Application【应用】 | Anti-GLUT-1 Antibody, C-terminus is an antibody against C terminus of GLUT-1 that is validated for the detection of GLUT-1 in Western Blotting (WB), Immunohistochemistry - Paraffin (IHC-P), Immunocytochemistry (ICC) and ELISA. Research Category Signaling Research Sub Category Insulin/Energy Signaling Immunohistochemisty (paraffin): GLUT-1 representative staining pattern/morphology on colorectal carcinoma. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Note: can be use as internal control for IHC run. Immunocytochemistry: Recommended ELISA: Recommended Western Blot: A previous lot of this antibody was tested on multiple lysates using western blot analysis. See datasheet. |
Quality【质量】 | Evaluated by Western Blotting in HUVEC. Western Blotting Analysis: 1 µg/mL of this antibody detected GLUT-1, CT in HUVEC. |
Linkage【联系】 | Replaces: AB1340 |
Physical form【外形】 | Purified rabbit polyclonal IgG in buffer containing 0.02 M Phosphate Buffer, 0.25 M NaCl, with 0.1% sodium azide. Affinity purified |
Analysis Note【分析说明】 | Control Jurkat Cell Lysate |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
purified by【纯化方式】 | affinity chromatography |
species reactivity | mouse, human |
species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
isotype【同位素/亚型】 | IgG |
NCBI accession no.【NCBI登记号】 | NM_006516 |
UniProt accession no.【UniProt登记号】 | P11166 |
shipped in【运输】 | wet ice |
产品说明
Target description【目标描述】 | This antibody detects a major band ~54 kDa in Western blot analysis. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at 2-8°C from date of receipt. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 2 |