Anti-PDGF-AA Antibody

32160702

Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin.

Reacts with PDGF-AA

Highly pure (>98%) recombinant human PDGF-AA.

Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
Detect PDGF-AA using this Anti-PDGF-AA Antibody validated for use in IH(P), ELISA, WB, NEUT.
Immunohistochemisty (paraffin): PDGF representative staining pattern/morphology in prostate cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

ELISA: 0.5 μg/mL dilution of a previous lot allowed detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-AA is used to coat the plate.

Neutralization: To yield ½ maiximal inhibition (ND50) of the biological activity of hPDGF-AA (5.0 ng/mL), a concentration of 0.05-0.07 μg/mL of a previous lot of this antibody was required.

Optimal working dilutions must be determined by the end user.

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PDGF-AA on 10 μg of C2C12 lysates.

Replaces: AB1486P

Affinity purified
Purified rabbit polyclonal IgG lyophilized from PBS, pH 7.2.

Control
C2C12 cell lysate

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

mouse, human

ELISA: suitable
immunohistochemistry: suitable (paraffin)
neutralization: suitable
western blot: suitable

IgG

NM_002607.4

P04085

dry ice

human ... PDGFA(5154)

24 kDa detected in Western Blot.

The lyophilized antibody is stable at room temperature for at least one month and for up to one year when kept at -20ºC. When reconstituted in sterile water to a concentration of 0.1-1.0 mg/mL, the antibody is stable for at least six weeks at 2-8ºC.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

13 - Non Combustible Solids

WGK 3