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Anti-phospho-Smad3 (Ser423/425) Antibody
产品别名
Anti-phospho-Smad3 (Ser423/425) Antibody
MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mothers against DPP homolog 3 , SMA- and MAD-related protein 3, SMAD 3 , SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD, mothers
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
| General description【一般描述】 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. |
| Specificity【特异性】 | Detects phosphorylated Smad3 (Ser423 and Ser425). |
| Immunogen【免疫原】 | KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3. Epitope: Phosphorylated Ser423/425 |
| Application【应用】 | Research Category Signaling Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB. |
| Quality【质量】 | Evaluated by western blot on untreated and UV-treated AKR-2B cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect Smad3 in UV-treated AKR-2B cell lysate. |
| Physical form【外形】 | Unpurified rabbit serum in buffer containing 0.05% sodium azide. |
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | unpurified |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| species reactivity | mouse, mink |
| species reactivity (predicted by homology) | human, rat |
| technique(s) | western blot: suitable |
| NCBI accession no.【NCBI登记号】 | NM_005902 |
| UniProt accession no.【UniProt登记号】 | P84022 |
| shipped in【运输】 | wet ice |
产品说明
| Target description【目标描述】 | 50 kDa |
| Storage and Stability【储存及稳定性】 | Stable for 2 years at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. |
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
