Anti-dimethyl-Histone H3 (Lys9) Antibody, Alexa Fluor^® 488 Conjugate

32160702

NA.41

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Histone H3 dimethylated on lysine 9.

Epitope: H3K9me2
KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.

Research Sub Category
Nuclear Receptors
Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells.
The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Protein A purified

Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a registered trademark of Life Technologies

rabbit

100

ALEXA FLUOR^® 488

purified antibody

primary antibodies

polyclonal

human, mouse

yeast (based on 100% sequence homology), rat (based on 100% sequence homology), chicken (based on 100% sequence homology)

immunocytochemistry: suitable

NM_003493

Q16695

wet ice

~17 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

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12 - Non Combustible Liquids

WGK 2